Quantification of Enterovirus RNA in Sludge Samples Using Single Tube Real-Time RT-PCR
نویسندگان
چکیده
منابع مشابه
Quantification of enterovirus RNA in sludge samples using single tube real-time RT-PCR.
We have developed a quantitative RT-PCR method that can be used to determine the amount of enterovirus RNA in urban sludge samples. This method combines Taq-Man technology with the ABI Prism 7700 real-time sequence detection system. We optimized a one-step RT-PCR that uses a dual-labeled fluorogenic probe to quantify the 5' noncoding region of enteroviruses. For accurate quantification of the n...
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BACKGROUND Circulating tumour cells (CTCs) are cells that have detached from a primary tumour, circulate in the peripheral blood, and are considered to be the main root of distant metastases. We present a method for the detection of CTCs by real-time PCR on different cytokeratin markers. MATERIALS AND METHODS Blood samples of a healthy donor were mixed with specific numbers of cells from diff...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2000
ISSN: 0736-6205,1940-9818
DOI: 10.2144/00291st03